β-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278
نویسندگان
چکیده
Abstract Background Our previous study shows that LINC01278 inhibits the malignant proliferation and invasion of papillary thyroid carcinoma (PTC) cells by regulating miR-376c-3p/DNM3 axis. However, regulation mechanism expression in PTC is still unclear. Methods The luciferase reporter ChIP assays were used to confirm binding LEF-1 putative promoter site gene. RNA immunoprecipitation pulldown determine enrichment β-catenin protein. proteasome inhibitors (MG132) was for detecting ubiquitination-proteasome degradation. Wnt/β-catenin specific agonists (LiCI), (WiKI4) TOP/FOP-flash assay activation signal. Western blot detected target proteins. Results online PROMO algorithm determines a on promoter, binds gene, enhances gene promoter. Furthermore, negatively regulated protein accumulation cytoplasm, into nucleus, ultimately inhibited transcription downstream genes activated results proved direct In addition, combination promotes Conclusion summary, we found transcriptional β-catenin/LEF-1 factor, negative feedback onβ-catenin
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ژورنال
عنوان ژورنال: Cancer Cell International
سال: 2021
ISSN: ['1475-2867']
DOI: https://doi.org/10.1186/s12935-021-02082-9